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inositol free dmem  (Valiant Co Ltd)

 
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    Valiant Co Ltd inositol free dmem
    Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing <t>DMEM.</t> p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).
    Inositol Free Dmem, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 931 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inositol+free+dmem/DMEM/pmc13129382-268-20-22
    Average 96 stars, based on 931 article reviews
    inositol free dmem - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "The inositol pyrophosphate 5-InsP 7 regulates mitochondrial polyphosphate synthesis and bioenergetic function"

    Article Title: The inositol pyrophosphate 5-InsP 7 regulates mitochondrial polyphosphate synthesis and bioenergetic function

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111413

    Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing DMEM. p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).
    Figure Legend Snippet: Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing DMEM. p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).

    Techniques Used: Activity Assay, Cell Culture, Two Tailed Test, Phospho-proteomics, Control, Fluorescence, Isolation, Incubation



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    inositol free dmem  (Valiant Co Ltd)
    96
    Valiant Co Ltd inositol free dmem
    Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing <t>DMEM.</t> p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).
    Inositol Free Dmem, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inositol+free+dmem/DMEM/pmc13129382-268-20-22
    Average 96 stars, based on 1 article reviews
    inositol free dmem - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing DMEM. p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).

    Journal: The Journal of Biological Chemistry

    Article Title: The inositol pyrophosphate 5-InsP 7 regulates mitochondrial polyphosphate synthesis and bioenergetic function

    doi: 10.1016/j.jbc.2026.111413

    Figure Lengend Snippet: Effect of mitochondrial activity on polyP synthesis. A , bar graph depicting mitochondrial polyP levels (mean ± S.D.) in U-2 OS cells cultured in high-glucose (25 mM), galactose (10 mM), or low-glucose (5 mM) containing DMEM. p values were determined using a two-tailed unpaired Student's t test (N = 3) B-C , bar graphs representing mitochondrial polyP levels ( B ) and whole cell ATP levels ( C ) in U-2 OS cells treated for 3 h with Antimycin A (2 μM; complex III inhibitor), FCCP (100 nM; protonophore and uncoupler of oxidative phosphorylation), Oligomycin (100 ng/ml; complex V inhibitor), or DMSO (vehicle control). p values were determined using a two-tailed unpaired Student's t test. All the experiments were performed at least three times. D-G , line graphs representing the fold change in DAPI fluorescence over time as a measure of polyP synthesis in isolated mitochondria incubated with glutamate (5 mM), malate (5 mM), and succinate (10 mM), under different conditions as indicated: high Pi (5 mM) with varying concentrations of ATP ( D ); high ATP (1 mM) with varying concentrations of Pi ( E ); addition of ATP (1 mM), non-hydrolyzable ATP analog AMP-PNP (1 mM), or Pi (1 mM), along with 5 mM Pi ( F ); Pi (5 mM) and ATP (1 mM), along with mitochondrial inhibitors at the indicated concentrations, or DMSO as a control ( G ).

    Article Snippet: Cells seeded in 60 mm dishes in normal growth medium were allowed to attain 30% confluence and then transferred to inositol-free DMEM (MP Biomedicals, D9802-06.25) containing 10% dialyzed FBS and labeled with 40 μCi myo -2-[ 3 H] inositol for 2.5 days.

    Techniques: Activity Assay, Cell Culture, Two Tailed Test, Phospho-proteomics, Control, Fluorescence, Isolation, Incubation